Circular Dichroism and Linear DichroismThis book provides an introduction to all those who wish to use the complementary spectroscopic techniques of optical activity (circular dichroism, CD) and optical anisotropy (linear dichroism, LD) for the study of the structure of molecules and interactions between molecules in solution. It emphasizes these techniques and how to use them for both low and high molecular weight molecules. The book begins by describing the principles behind CD and LD and how these techniques can be used in the laboratory without using advanced maths or quantum mechanics. The next chapters describe how both techniques may be applied to the study of biological macromolecules and give a detailed description of how they may be used on small molecules to investigate molecular and electronic structure. The final part contains theoretical derivations of all the equations required for the applications described previously. Specific molecular examples are used to illustrate concepts and to show the reader how to use the techniques in chemical and biological systems. Circular Dichroism and Linear Dichroism is an easy guide to what a prospective user of CD needs to know and explains how LD is not merely an exotic technique only to be practiced by experienced spectroscopists, but may be routinely and usefully employed as an aid to molecular structure determination. |
Contents
Spectroscopy chirality and oriented systems | 1 |
Circular dichroism of biomolecules | 15 |
Linear dichroism of biomolecules | 32 |
Linear dichroism of small molecules | 45 |
33 | 85 |
magnetic | 90 |
44 | 93 |
47 | 120 |
65 | 126 |
References | 131 |
Common terms and phrases
absorbance achiral achiral chromophore angle atoms axis system B-DNA base pairs binding bond bra-ket notation carbonyl CD induced CD signal CD spectrum CD strength Chapter Chem chiral chromophore Circular dichroism circularly polarized light component cos² coupled-oscillator CD coupling d-d transitions defined degenerate coupled-oscillator determine dipole transition direction E band eda transition edtm electric dipole electric field electron enantiomer exciton factor geometry helical helix axis illustrated in Fig interaction isotropic ligand Linear dichroism linearly polarized light long axis magnetic field magnetic moment magnitude mdtm measure metal complexes molecule negative non-degenerate Nordén octant rule orbital orientation parameters parallel peptide perpendicular perturbation planar plane polarizability protein radiation right circularly polarized Rodger sample solution spectra spectroscopy structure substituent symmetry tetracene transition energy transition moments transition polarizations usually vector wavefunctions wavelength Wavelength/nm Z-DNA z-polarized