Principles of Fluorescence Spectroscopy

Front Cover
Springer Science & Business Media, Dec 5, 2007 - Science - 954 pages

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

 

Contents

TimeResolved Energy Transfer
478
Energy Transfer to Multiple Acceptors
507
Protein Fluorescence
529
9
535
Quenching
547
41
552
280
577
Anisotropy Decay of Ribonuclease
585

BeerLambert
58
1
60
7
64
5
66
10
81
144
85
TimeDomain Lifetime Measurements
91
3
99
6
105
DNA Sequencing
123
7
161
4
183
Decay Rates
222
Overview of ExcitedState Processes
237
9
243
33
245
Quenching of Fluorescence
277
10
281
34
297
Applications of Quenching to Molecular
310
2
315
257
326
4
328
Mechanisms and Dynamics
332
37
335
Effects
343
Fluorescence Anisotropy
353
References
378
364
393
Advanced Anisotropy Concepts
413
7
424
40
437
44
598
315
605
Multiphoton Excitation and Microscopy
608
Fluorescence Sensing
623
281
660
316
664
317
715
Quenching by Gold
723
45
726
FluorescenceLifetime Imaging Microscopy
741
6
744
SingleMolecule Detection
757
46
768
318
795
Fluorescence Correlation Spectroscopy
797
13
803
Detection of Conformational Dynamics
820
49
851
52
858
Corrected Emission Spectra
873
327
877
Additional Reading
889
Index
923
805
925
477
929
812
933
332
934
338
942
195
943
626
948
363
949
Copyright

Other editions - View all

Common terms and phrases

About the author (2007)

Dr. Joseph R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine, Baltimore, and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the author of the widely used text, Principles of Fluorescence Spectroscopy now in its 3rd edition.

Bibliographic information