Near-Infrared Applications in BiotechnologyThis volume explores developments in techniques in diagnostics, DNA sequencing, bioanalysis of immunoassays, and single-molecule detection. It promotes the measurement, identification, monitoring, analysis, and application of near-infrared spectroscopy (NIR) to medical and pharmaceutical advances. The text also considers noninvasive methods of NIR for successful, cost-effective, and prompt diagnoses of diseases. |
Contents
Introduction | 1 |
Some Aspects of Fluorescence of Particular Relevance for Biological Processes | 5 |
Probes Dyes Fluorescing in the NIR Region | 35 |
Analytical Applications of NearInfrared Fluorescence in Immunoassays | 95 |
NearInfrared Applications in DNA Sequencing and Analysis | 133 |
NearInfrared Applications in Medicine | 151 |
NearInfrared Applications in SingleMolecule Detection | 185 |
Applications Using Fluorescence Lifetimes | 201 |
Applications in Polymers | 235 |
Beyond Biotechnology and into Popular Technology | 263 |
Fundamentals of NearInfrared Spectroscopy | 293 |
Biomedical Applications of NearInfrared Spectroscopy | 323 |
Pharmaceutical Applications of NearInfrared Spectroscopy | 347 |
Index | 365 |
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Common terms and phrases
absorbance Anal Chem analysis analyte antibody antigen Appl Spectrosc applications assay band blood calibration cells Chem chemical chemistry chromophores concentration conjugate curve cyanine cyanine dyes decay deep red detection detector determined developed diode laser distribution DNA sequencing dye lasers dye molecules electronic emission energy excitation Figure fluorescence lifetime fluorophores functional dye G Patonay glucose groups imaging immunoassay infrared instrument intensity interactions labeled light materials matrix Matsuoka measurements methine method molecular molecules near-infrared spectroscopy NIR dyes NIR fluorescence NIR region NIR spectroscopy optical organic dyes oxygen Photochem Photobiol photodynamic therapy photon phthalocyanine Phys polymer polymeric polymethine chain polymethine dyes porphyrins probes properties protein pulse quantum yield radiation Raman Raman scattering reaction samples scattering sensitivity shift signal single molecules singlet solution solvent spectral region spectrum Stokes shift structure synthesis tablets technique temperature time-resolved tissue transition tricarbocyanine values wavelength
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