Experiments in Molecular Genetics

A(lacpro Acad aeration agar amber arabinose assay bacterial Bacteriol Biol buffer cells cells/ml chromosome Cold Spring Harbor containing Day 1 Day deletions dilution tubes donor enzymes episome Escherichia coli Experiment F factor F'lac Figure flask fresh overnight gene genetic glucose minimal plates grow growth Hfr strains HfrC HfrH histidine Incubate at 37 indicator plates induced interrupted mating IPTG isolated Lac mutants lac operon lac region lac repressor lacpro lactose lactose minimal plates lactose tetrazolium plates LB plates lysate lysogens markers mating mixture Method Day mg/ml minutes ml LB broth mutagen mutagenesis mutants nalidixic acid nonsense mutations overnight culture pasteur pipettes Phen phenotype proline prophage protein recipient recombinants replication resuspend revertants round wooden toothpicks selective plates single colonies ẞ-galactosidase strA Strains Number Sex streptomycin suppressor synthesis temperature temperature-sensitive test tubes transducing phage transfer tryptophan waterbath Xgal µg/ml