Introduction to Animal Cytogenetics

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Springer Science & Business Media, Jul 31, 1993 - Medical - 238 pages
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This book presents animal cytology as a science of seeing and interpreting chromosome form and behaviour, and of appreciating its evolutionary significance. Its principal objective is to help students develop a basic understanding and confidence on all matters relating to animal chromosomes.
 

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Contents

Chromosomes in mitosis
1
THE CELL CYCLE
2
CHROMOSOME NUMBERS
3
CHROMOSOME SHAPE
4
CHROMOSOME SIZE
6
CHROMOSOMES IN MITOSIS
7
DNA PER NUCLEUS CVALUE
9
RECOMMENDED FURTHER READING
12
VARIATION AND EVOLUTION
134
THE ACTIVITY OF NUCLEOLUS ORGANIZERS
136
RECOMMENDED FURTHER READING
138
Lampbrush chromosomes
139
BASIC ORGANIZATION
143
TWO FAMOUS HYPOTHESES
146
LAMPBRUSHES UNDER THE ELECTRON MICROSCOPE
147
IN SITU HYBRIDIZATION
149

Karyology and evolution
13
CHROMOSOME BANDING
16
KARYOTYPES GENOMES AND EVOLUTION
21
RECOMMENDED FURTHER READING
28
Meiosis
29
TIMING AND SEQUENCE
30
THE SYNAPTONEMAL COMPLEX
45
CHROMOSOME PAIRING
48
CHIASMATA AND CROSSING OVER
54
DISJUNCTION
58
DURATION OF MEIOSIS
63
MEIOSIS IN REARRANGED CHROMOSOMES
64
RECOMMENDED FURTHER READING
69
Sex chromosomes and sex determination
71
RECOMMENDED FURTHER READING
76
Polyploidy in animals
77
SOME SPECIFIC EXAMPLES
79
EVOLVING POLYPLOIDY
81
RECOMMENDED FURTHER READING
83
Inactivation and elimination of chromosomes
85
X INACTIVATION IN MICE AND MULES
87
THE ROLE OF THE SECOND X
88
THE GENETIC AND MOLECULAR BASIS OF INACTIVATION
90
MORE ABOUT INSECTS
95
ASCARIS AND CHROMATIN ELIMINATION
96
THE PROTOZOAN MACRONUCLEUS
100
RECOMMENDED FURTHER READING
102
DNA replication endomitosis and trophic polyploidy
103
THE PRINCIPLES OF TROPHIC POLYPLOIDY
104
SOME EXAMPLES OF POLYPLOID CELLS
106
POLYTENE CHROMOSOMES
110
SELECTIVE DNA REPLICATION
113
RECOMMENDED FURTHER READING
117
The nucleolus organizer and gene amplification
119
IDENTIFYING NUCLEOLUS ORGANIZERS
121
RINGS CHRISTMAS TREES AND TRANSCRIPTION UNITS
124
UNDERSTANDING AMPLIFICATION
130
THE READTHROUGH HYPOTHESIS
152
THREE SIMPLE EXPERIMENTS WITH ENZYMES
153
SOME MORE QUESTIONS ABOUT LAMPBRUSHES
157
RECOMMENDED FURTHER READING
160
DNA sequences and genome evolution
161
VARIATION IN REPEAT SEQUENCE DNA
162
AGENTS OF CHANGE
164
SISTER CHROMATIC EXCHANGE
166
WHAT KEEPS GENOMES IN CHECK?
169
THE STORY OF A LIBERATED CHROMOSOME
172
RECOMMENDED FURTHER READING
174
Human clinical cytogenetics
177
THE PROBLEM OF ANEUPLOIDY
181
TRISOMY 21 AND DOWNS SYNDROME
183
MONOSOMY X
185
THE CLINICAL CYTOGENETICS LABORATORY
186
RECOMMENDED FURTHER READING
191
Outlook for 2001
193
A selection of laboratory practical protocols
201
SOME NOTES ON LIGHT MICROSCOPY
202
THE MITOTIC CHROMOSOME
205
NUCLEAR SEXING FROM ORAL MUCOSAL SMEARS
208
NUCLEAR SEXING FROM POLYMORPHONUCLEAR LEUCOCYTES NEUTROPHILS
209
INFORMATION FOR INSTRUCTORS AND TECHNICAL STAFF
210
CELL SIZE DNA CONTENT AND POLYPLOIDY
213
POLYTENE CHROMOSOMES
215
INFORMATION FOR INSTRUCTORS AND TECHNICAL STAFF
218
MEIOSIS
221
NEWTS AND SALAMANDERS
223
INFORMATION FOR INSTRUCTORS AND TECHNICAL STAFF
224
LAMPBRUSH CHROMOSOMES
226
ISOLATION OF THE CHROMOSOMES AND NUCLEOLI
227
OBSERVATION
228
INFORMATION FOR INSTRUCTORS AND TECHNICAL STAFF
229
Species index
233
Index
235
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