Preparative Liquid Chromatography
Elsevier, Jul 1, 1987 - Science - 340 pages
This volume provides a straightforward approach to isolation and purification problems with a thorough presentation of preparative LC strategy including the interrelationship between the input and output of the instrumentation, while keeping to an application focus.
The book stresses the practical aspects of preparative scale separations from TLC isolations through various laboratory scale column separations to very large scale production. It also gives a thorough description of the performance parameters (e.g. throughput, separation quality, etc.) as a function of operational parameters (e.g. particle size, column size, solvent usage, etc.). Experts in the field have contributed a well balanced presentation of separation development strategies from preparative TLC to commercial preparative process with practical examples in a wide variety of application areas such as drugs, proteins, nucleotides, industrial extracts, organic chemicals, enantiomers, polymers, etc.
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Chapter 2 Preparative Thin Layer Chromatography
Chapter 3 Increasing the Efflciency of Very Large Scale Packed Bed Chromatographic Separations
Chapter 4 Preparative Liquid Chromatography in the Pharmaceutical Industry
Chapter 5 Preparative Liquid Chromatography for the Synthetic Chemist
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acetate acetonitrile adsorbent adsorption amino acids amount Anal analytical column analytical LC analytical separation applications band baseline Biochem Chem chemical chiral Chromatogr column packing compounds containing counter-current decrease detector diameter effect efficiency eluent elution chromatography elution volume enantiomeric enantiomers equilibration flow rate fraction collection gradient grams hexane increased injection volume interactions ion exchange isolation laboratory larger layer Liquid Chromatography methanol methods mixture mL/min mobile phase molecules obtained optimal overload packed bed packing material particles peak shape peptide polar polymer pore preparative LC preparative LC separation preparative LC systems preparative liquid chromatography preparative SEC preparative separations pressure protein PTLC pump purity racemate recovery recycle resolution resolved retention reversed phase sample components sample concentration sample load Section semi-preparative separation factor shown in Fig silica gel solute solvent stationary phase surface synthetic technique theoretical plates throughput trifluoroacetic acid values zone