Protein and Pharmaceutical Engineering: Proceedings of a UCLA Symposium Held at Park City, Utah January 17-22, 1989Charles S. Craik Protein and Pharmaceutical Engineering brings together a distinguished group of experts who scrutinize the architecture and activity of proteins to uncover the underlying principles of macromolecular form and function. Drawing on this knowledge, the contributors to this volume focus on designing and engineering proteins for the development of pharmaceuticals. The authors employ novel approaches in molecular genetics, enzymology, molecular modelling, NMR spectroscopy and X-ray crystallography to reveal the role primary amino acid sequence plays in protein structure and function. Chapters cover areas of research in two broad categories: physical analysis of protein structure and molecular biochemical aspects of protein function. |
Contents
Robert O Fox Department of Molecu Patrick Loll Department of Biophysics | 1 |
Detection of Conformational Changes in Active Site Mutants | 17 |
Combining Sites and Epitopes Defined by Molecular Modeling Protein | 35 |
Copyright | |
10 other sections not shown
Common terms and phrases
43kD protein Acad Sci USA AChR active active site amines amino acid analog analysis antibody combining antigen aspartate aspartate transcarbamoylase assay binding proteins Biochem Biochemistry Biol Chem bond Breslow buffer carboxylate catalysis catalyzed cells Chem Soc chemical shift cleavage complex concentration conformational changes crystal structure cyclization database determined disulfide domain enantiomers encoded enzyme epitope expressed Figure function gene glutathione reductase H NMR histidine HIV1 protease human immunodeficiency virus hydrophobic inhibitor interactions ion channel ketimine kinetic lactone leucine ligand LIV-BP loops membrane molecular molecules mutant Natl Acad Sci NHAC nicotinic acetylcholine receptor nuclease phosphate plasmid polyacrylamide gels predicted Proc Natl Acad proton purified protease rate constant rate-determining reaction region residues sample sequence shown side chain site-directed mutagenesis spectra Staphylococcal nuclease Stroud RM subunit synthetic peptides Torpedo transamination transition transport triosephosphate isomerase viral wild type SNase wild-type enzyme x-ray structure