Working with Animal Chromosomes
Wiley, Dec 26, 1988 - Science - 290 pages
A guide to handling giant polytene and lampbrush chromosomes, preparing chromatin, visualizing transcription, locating genes on chromosomes, identifing fine-scale polymorphisms on mitotic chromosomes, etc. Covers many specialized chromosome techniques; each method is tested and reproducible and comes with a model protocol. This 2nd Edition has several new sections on recently developed cytological techniques including spreading of synaptonemal complexes from cells in meiotic prophase, banding of mitotic chromosomes with Giemsa and fluorochromes, indirect immunofluorescence, lampbrush chromosomes from Xenopus laevis, and isolation of polytene chromosomes. Also covers new legislation affecting those who work with animal chromosomes.
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acetic acid activity allow amount animal antibodies applied banding blood buffer cells centrifuge chamber Chapter chromatin Chromosoma Berl clean complexes concentration containing contents contrast coverglass culture cytoplasm described dispersal dissecting distilled water drop Drosophila effect emulsion et al ethanol examined Figure filter final fixative gene gently Giemsa given glands human hybridization hypotonic immediately important incubation isolation labelled laboratory lampbrush chromosomes Leave length light material measuring medium meiotic metaphase method microscope Miller needed normal nuclear nuclei objective observation obtained oocyte pairs phase piece pipette plastic polytene chromosomes possible preparations probe procedure produced proteins protocol remove Rinse sequences slide solution species spread squash stage staining step structure studies suitable surface suspension technique Telephone temperature testis tissue transcription transfer treatment units usually wash