Antibody Engineering

Front Cover
Carl A. K. Borrebaeck
Oxford University Press, 1995 - Medical - 390 pages
As the field of antibody engineering continues its explosive growth, more and more scientists around the world are striving to keep up with the latest developments. Now in its second edition, Antibody Engineering is the perfect one-stop introduction to state-of-the-art technologies emerging in the field today. In presenting a practical overview of the engineering of recombinant human or mouse monoclonal antibodies, the book incisively addresses essential topics such as antibody structure relevant to antibody engineering, recombinatorial cDNA libraries, phage display, synthetic and humanized antibodies, engineering of affinity and biological effector functions, and plant, mammalian, and bacterial expression vectors and hosts. Antibody Engineering, Second Edition--written by leading experts and now thoroughly updated--is a unique resource for current information on the subject. It will be welcomed by researchers in immunology, biotechnology, molecular biology, and biochemistry.
 

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Contents

Antibody Structure and Function
3
Human Monoclonal Antibodies from VGene Repertoires
53
Creation of scFv Antibody Fragment Gene Repertoires
61
Phage Display Can Be Used to Make Antibodies Without Immunization
68
Rescue of Phagemid Particles for Selection on Antigen
81
Methods
104
Engineering the Antibody Combining Site by CodonBased
117
Human Metaphoric Antibodies from a Phagemid Library
133
Expressing Antibodies in Escherichla Coll
229
Vectors and Approaches for the Eukaryotic Expression
267
Vectors for the Expression of IgFusion Proteins
280
The Cloning of Hybridoma V Regions for Their Ectopic
295
Ectopic Expression of Cloned V Regions for Intracellular and Intercellular
330
Working Examples
345
The Integrated Vector System
353
The Expression of Single Chain Antibodies
363

Stragles for Humanizing Antibodies
159
SingleChain Fv Design and Production by Preparative Folding
185
Culture Supernatant for ScFv Secretion
371
Copyright

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Page 375 - An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46: 291-300.

About the author (1995)

Carl A.K. Borrebaeck is at University of Lund.

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