Antibodies: A Laboratory Manual

Front Cover
ince its publication in 1988, Antibodies: A Laboratory Manual, by Harlow and Lane, has become a classic, an essential resource for molecular biology, immunology, and cell culture labs. In order to keep the book in print, Cold Spring Harbor Laboratory Press eventually produced the paperback edition currently available for sale.

Now, after 25 years, a second edition is being published in September 2013. Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added. The new edition provides clear, authoritative, current and up-to-date protocols with background information and troubleshooting advice. The book is an invaluable resource for all those engaged in antibody research and development.
 

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I work for ImmunoReagents-a leader in polyclonal antibodies and we have referred to this book many times over the years. It gives a very broad view of many immuno-applications and techniques. We always keep it handy and use it with confidence when we manufacture our polyclonal antibodies. We have recently purchased a newer edition and find it equally useful. 

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how to prepere antigensa and antibodies
purifiation
sds-page

Contents

ANTIBODY RESPONSE
37
IMMUNOGENICITY
55
IMMUNIZING ANIMALS
92
MONOCLONAL ANTIBODIES
139
recognition site Techniques of cell fusion allow individual B cells to
148
GROWING HYBRIDOMAS
245
9
315
IMMUNOPRECIPITATION
424
N IMMUNOASSAY
553
15
613
Proteolytic Fragments of Antibodies
626
ADSORPTION TO REMOVE NONSPECIFIC BINDING
632
Protein Techniques
658
Appendix Ill General Information
682
Bacterial Expression
690
References
697

12
471
13
511
Index
711
Copyright

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Common terms and phrases

Popular passages

Page 694 - Western Blotting": Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated Protein A. Anal. Biochem.
Page 697 - KARNOVSKY, MJ: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
Page 704 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 694 - BRADFORD, MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Page 52 - Sideras P, Konishi M, Azuma C, Tominaga A, Bergstedt-Lindqvist S, Takahashi M, Matsuda F, Yaoita Y, Takatsu K, Honjo T: Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II. Nature 324:70, 1986 10.
Page 701 - Nakane, PK : Simultaneous localization of multiple tissue antigens using the peroxidaselabeled antibody method: A study on pituitary glands of the rat. J. Histochem. Cytochem.
Page 18 - Davies, D. R. (1974) The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site. Proc. Natl. Acad. Sci. USA 71, 4298^302.
Page 699 - K., 1979, A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibodysecreting hybrid cell lines, J.
Page 51 - EL (1983). Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex. J Exp Med 157:705.

About the author (1988)

Lane is Reader in Sociology and Fellow of Emmanuel College, Cambridge.

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