Methods in Alcohol-Related Neuroscience ResearchYuan Liu, David M. Lovinger Written by a panel of experts, Methods in Alcohol-Related Neuroscience Research not only provides information of a technical nature but also gives an overview of the many areas in investigating the effects of alcohol on the brain. It gives technical guidance for investigators doing research at the molecular, cellular, systems, and behavioral levels. These techniques include a wide variety of approaches, ranging from gene mapping and examination of molecular interactions of alcohol at the sub-cellular level to recording of neural activities in freely-behaving animals and imaging alcohol effects on the living human brain. |
Contents
Chapter 2 Knockout and Knockin Mice | 31 |
Appendix 21 | 57 |
Appendix 22 | 59 |
Chapter 3 Molecular Studies by Chimeragenesis and Mutagenesis | 63 |
Chapter 4 Combining PatchClamp Recording and Gene Profiling in Single Neurons | 83 |
Appendix 41 Protocol for SingleCell mRNA AmplificationReverse Northern Analysis | 99 |
Appendix 42 Protocol for SingleCell RTPCR | 109 |
Appendix 43 Protocol for SingleCell Multiplex RTPCR | 111 |
Chapter 7 Rapid Drug Superfusion and Kinetic Analysis | 159 |
Chapter 8 Electrophysiological Assessment of Synaptic Transmission in Brain Slices | 191 |
Chapter 9 Cognitive Correlates of Single Neuron Activity in TaskPerforming Animals | 219 |
Chapter 10 Multichannel Neural Ensemble Recording During Alcohol Self Administration | 241 |
Chapter 11 Combined Single Neuron Recording and Microdialysis in the Brain of Freely Behaving Animals Rodents and NonHuman Primates | 259 |
Chapter 12 QuantitativeMicrodialysis for in vivo Studies of Pharmacodynamics | 287 |
Chapter 13 Structural Magnetic Resonance Imaging MRI of the Human Brain | 319 |
Chapter 14 Biochemical Functional and Microstructural Magnetic Resonance Imaging MRI | 345 |
Other editions - View all
Methods in Alcohol-Related Neuroscience Research Yuan Liu,David M. Lovinger No preview available - 2002 |
Common terms and phrases
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