Antibodies in Cell BiologyAntibodies in Cell Biology focuses on a new generation of protocols aimed at the cell biologist. This laboratory manual features systems and techniques that are especially relevant for modern problems. The contributing authors have been carefully chosen for their specific expertise, and have provided detailed protocols, recipes, and troubleshooting guides in each chapter. The book is designed for any researcher or student who needs to use antibodies in cell biology and related research areas. Practical applications and future emphases of antibodies, including:
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Contents
| 7 | |
Chapter 3 Making Monoclonal Antibodies | 57 |
Chapter 4 Purification of Mouse Antibodies and Fab Fragments | 75 |
Chapter 5 BlotAffinity Purification of Antibodies | 95 |
Chapter 6 Immunoblotting | 105 |
Chapter 7 Immunoprecipitation Methods | 119 |
Chapter 8 A Quantitative SolidPhase Binding Assay for Tubulin | 129 |
Chapter 9 Confocal Immunofluorescence Microscopy of Microtubules in Oocytes Eggs and Embryos of Algae and Amphibians | 147 |
Characteristics and Use in Immunocytochemical Studies | 241 |
Mapping Tubulin Isotypes on Neurite Microtubules | 259 |
Chapter 15 Postembedding Immunocytochemical Techniques for Light and Electron Microscopy | 283 |
Chapter 16 Electron Microscopy Immunocytochemistry Following Cryofixation and Freeze Substitution | 311 |
Chapter 17 Microinjection of Antibodies in the Analysis of Cellular Architecture and Motility | 343 |
Chapter 18 Immunoselection and Characterization of cDNA Clones | 361 |
Methods Applications and Critique | 407 |
Appendix | 441 |
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Common terms and phrases
acid adjuvant anti antibody incubation antigen antiidiotypic antibodies antiserum assay autofluorescence binding blot buffer cDNA Cell Biol Cell Biology cell wall cellular centrifuge clones colloidal gold concentration confocal containing coverslip cryofixation culture cytoplasmic cytoskeletal dilution distilled H2O dynein electron microscopy ELISA elution embedding embryos epitope filter fixation fixative fluorescence freeze substitution fusion protein glutaraldehyde gold particles gold probes grids hybridomas immunization immunoblotting immunocytochemistry immunofluorescence immunofluorescence microscopy immunogen immunoglobulins immunogold immunolabeling immunoprecipitation injection isotypes labeling Leunissen ligand medium membrane methanol method microtubules molecular molecules monoclonal antibodies mouse NaCl nitrocellulose nonspecific obtained pellet peptide plaque plate polyclonal antibodies polypeptide primary antibody procedure protocol purified rabbit reagents remove resin Resuspend Rinse room temperature samples screening SDS-PAGE sea urchin secondary antibody sections sequence serum silver enhancement slides sodium specific specimen staining step techniques tissue titer tube tubulin uranyl acetate volume wash yeast
Popular passages
Page 404 - Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Page 436 - Benjamin DC, Berzofsky JA, East IJ, Gurd, FRN, Hannum C., Leach SJ, Margoliash E., Michael JG, Miller A., Prager EM, Reichlin M., Sercarz EE, Smith-Gill SJ, Todd PE, and Wilson AC 1984. The antigenic structure of proteins: A reappraisal. Annu. Rev. Immunol. 2: 67-101.
Page 436 - Chothia, C., Lesk, AM, Tramontano, A., Levitt, M., Smith-Gill, SJ, Air, G., Sheriff, S., Padlan, EA, Davies, D., Tulip, WR, Colman, PM, Spinelli, S., Alzari, PM, and Poljak, RJ (1989) Conformations of immunoglobulin hypervariable regions.
Page 436 - Atassi, MZ (1975). Antigenic structure of myoglobin: The complete immunochemical anatomy of a protein and conclusions relating to antigenic structures of proteins.
Page 438 - Padlan, EA, Silverton, EW, Sheriff, S., Cohen, GH, Smith-Gill, SJ, and Davies, DR (1989) Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex.
Page 439 - Crystal structures of an antibody to a peptide and its complex with peptide antigen at 2.8 A. Science 248, 712-719.
References to this book
Proteine: Standardmethoden der Molekular- und Zellbiologie: Präparation ... Werner A. Eckert,Jürgen Kartenbeck No preview available - 1997 |