Proteome Research: Two-Dimensional Gel Electrophoresis and Identification MethodsT. Rabilloud Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements. |
Contents
| 1 | |
Initial Solubilization | 14 |
TwoDimensional Electrophoresis with Carrier Ampholytes | 31 |
Modification of the Standard TwoDimensional Gel Method | 47 |
Sample Preparation | 65 |
Equilibration of IPG Strips | 80 |
Detection of Proteins on TwoDimensional Electrophoresis Gels | 107 |
Membrane Staining | 132 |
Identification by Amino Acid Composition Obtained from Labeling | 163 |
Double Labeling Method using Scintillation Counting | 170 |
Result Analysis | 176 |
Other Possibilities for Generation of Amino Acid Sequences | 186 |
Conclusion | 195 |
Enzymatic Digestion of Proteins | 201 |
Mass Spectrometers | 207 |
Protein Identification by Correlating MS DATA with Sequence | 216 |
Identification of Proteins by Amino Acid Composition | 142 |
Derivatization and Chromatography | 149 |
Protein Identification by Database Matching | 155 |
Mass Spectrometry of Intact Proteins from TwoDimensional PAGE | 233 |
UVMALDI Mass Spectrometry of Proteins Directly | 242 |
Other editions - View all
Proteome Research: Two-Dimensional Gel Electrophoresis and Identification ... T. Rabilloud No preview available - 1999 |
Proteome Research: Two-Dimensional Gel Electrophoresis and Identification ... T. Rabilloud No preview available - 2011 |
Common terms and phrases
2-D electrophoresis acrylamide amino acid amino acid analysis amino acid composition amino acid sequence ammonium persulfate ampholytes Anal Biochem antibodies applied blotting buffer carrier ampholytes cassette cathodic cells Chem chemical cleavage concentration Coomassie database deionized water derivatization detection detergents digestion Edman Edman degradation electroblotted electrode Electrophoresis 18 equilibration ethanol extraction fluorescent Fmoc gene genome glass plates Görg Hochstrasser DF hydrolysis hydrophobic IEF gel Immobiline in-gel ionization IPG gel strips IPG strips isoelectric focusing labeling laser MALDI MALDI-MS mass spectrometry matrix method molecular weight molecules MS/MS N-terminal nitrocellulose peptide pH gradients polyacrylamide gels polymerization polypeptides precipitation prepared procedure proteases protein identification proteome protocol PVDF Rabilloud reagent rehydration reswelling Rinse sample SDS gel SDS-PAGE second dimension sensitivity separation sequence tag silver staining slab gel sodium solubilization solution solvent spectra spots sulfate technique teins tion transfer tube two-dimensional electrophoresis two-dimensional gel electrophoresis urea voltage