Yeast Gene Analysis

Front Cover
Elsevier, Sep 21, 2011 - Science - 730 pages
Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.
  • New chapters on: Strain construction; genome-wide two-hybrid approaches; use of microarrays for transcript analysis; real-time analysis of chromosome behaviour and FRET; synthetic gene array technology and protein arrays; chemical genomics and yeast prions; RNA gene analysis and mitochondrial gene function analysis; phylogenetic footprinting; discovering human gene function and predicting yeast gene function
 

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Contents

Chapter 3 Yeast Transformation
45
Chapter 4 A Guided Tour to PCRbased Genomic Manipulations of S cerevisiae PCRtargeting
55
Generating and Using Promoter Fusions and Conditional Alleles
79
Chapter 6 Yeast Hybrid Approaches
103
Chapter 7 ArrayBased Yeast TwoHybrid Screening for ProteinProtein Interactions
139
Chapter 8 Reporter Genes and Their Uses in Studying Yeast Gene Expression
165
A Microarray Approach
189
Chapter 10 GFPbased Microscopic Approaches for Whole Chromosome Analysis in Yeasts
221
Chapter 17 Chemical Genomic Tools for Understanding Gene Function and Drug Action
387
Chapter 18 RNA Gene Analysis
415
Chapter 19 Analysis of Gene Function of Mitochondria
445
Chapter 20 Yeast Prions and Their Analysis In Vivo
491
Chapter 21 Metabolic Control in the Eukaryotic Cell a Systems Biology Perspective
527
Chapter 22 Phylogenetic Footprinting
551
Chapter 23 Saccharomyces cerevisiae as a Tool for Human Gene Function Discovery
577
Chapter 24 Bioinformatic Prediction of Yeast Gene Function
597

Chapter 11 Immunological Methods
241
Chapter 12 Measuring the Proximity of Proteins in Living Cells by Fluorescence Resonance Energy Transfer between CFP and YFP
269
Chapter 13 Identification Characterization and Phenotypic Analysis of Covalently Linked Cell Wall Proteins
281
Chapter 14 Yeast Protein Microarrays
303
Chapter 15 Smart Genetic Screens
331
Chapter 16 HighThroughput Strain Construction and Systematic Synthetic Lethal Screening in Saccharomyces cerevisiae
369
Chapter 25 Yeast Genetic Strain and Plasmid Collections
629
The Remaining Challenges
667
Index
685
Colour Plate Section can be found at the back of this book
701
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About the author (2011)

Stark is the former host of "Pure Rock Talkback" on the groundbreaking metal station KNAC-FM, Long Beach, California.

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