Recombinant Gene Expression: Reviews and Protocols

Front Cover
Paulina Balbás, Argelia Lorence
Springer Science & Business Media, 2004 - Science - 506 pages
1 Review
In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.
 

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Contents

Host Cell Compatibility in Protein Expression
3
Production of Recombinant Proteins Challenges and Solutions
15
FoldingPromoting Agents in Recombinant Protein Production
53
PROKARYOTES
76
Back to Basics pBR322 and Protein Expression Systems in E coli
77
αComplementationEnabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experim...
91
Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
101
Overexpression of Chromosomal Genes in Escherichia coli
113
HighThroughput Expression in Microplate Format in Pichia pastoris
277
Multiple Gene Expression by Chromosomal Integration and CREloxPMediated Marker Recycling in Saccharomyces cerevisiae
287
Three Decades of Fungal Transformation Key Concepts and Applications
297
Three Decades of Fungal Transformation Novel Technologies
315
PLANTS AND PLANT CELLS
326
Gene Transfer and Expression in Plants
329
Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors
351
Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens
365

Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli
123
Plasmid Vectors for MarkerFree Chromosomal Insertion of Genetic Material in Escherichia coli
135
CopyControl pBACoriV Vectors for Genomic Cloning
145
CopyControl Tightly Regulated Expression Vectors Based on pBACoriV
155
CellFree Protein Synthesis With Prokaryotic Combined TranscriptionTranslation
169
Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae
183
Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria
209
Recombinant Protein Production in Antarctic GramNegative Bacteria
225
FUNGI
238
Recombinant Protein Production in Yeasts
241
Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae
259
HighThroughput Expression in Microplate Format in Saccharomyces cerevisiae
267
New Selection Marker for Plant Transformation
385
Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants
397
ANIMALS AND ANIMAL CELLS
416
Gene Transfer and Expression in Mammalian Cell Lines and Transgenic Animals
417
Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines
435
Inducible Gene Expression in Mammalian Cells and Mice
451
FlpMediated Integration of Expression Cassettes into FRTTagged Chromosomal Loci in Mammalian Cells
467
Generation of HighRecombinantProteinProducing Chinese Hamster Ovary CHO Cells
477
Preparation of Recombinant Proteins in Milk
485
Index
495
Copyright

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