Molecular Cloning: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction |
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Page v
... Plasmid DNA by Boiling Lysis ( Protocols 4 and 5 ) Introduction to Preparation of Plasmid DNA by Alkaline Lysis with SDS ( Protocols 1-3 ) 1 Preparation of Plasmid DNA by Alkaline Lysis with SDS : Minipreparation 2 Preparation of ...
... Plasmid DNA by Boiling Lysis ( Protocols 4 and 5 ) Introduction to Preparation of Plasmid DNA by Alkaline Lysis with SDS ( Protocols 1-3 ) 1 Preparation of Plasmid DNA by Alkaline Lysis with SDS : Minipreparation 2 Preparation of ...
Page 9-33
... plasmid DNA twice with phenol : chloroform . However , if RNase was added to the plasmid at a late stage in the purification process , i.e. , after depro- teinization , it should be removed by treatment with proteinase K , as follows ...
... plasmid DNA twice with phenol : chloroform . However , if RNase was added to the plasmid at a late stage in the purification process , i.e. , after depro- teinization , it should be removed by treatment with proteinase K , as follows ...
Page 13-29
... plasmid DNA from ~ 2.5 ml of the overnight culture ( please see Chapter 1 , Protocol 1 ) . 11. Digest the plasmid DNA prepared in Step 10 with the selective restriction enzyme : plasmid DNA 10x restriction enzyme buffer unique site ...
... plasmid DNA from ~ 2.5 ml of the overnight culture ( please see Chapter 1 , Protocol 1 ) . 11. Digest the plasmid DNA prepared in Step 10 with the selective restriction enzyme : plasmid DNA 10x restriction enzyme buffer unique site ...
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agarose gel aliquots amplified DNA annealing antibody Appendix bacteriophage T4 Biol BioTechniques catalyzed cDNA clones cDNA libraries cDNA synthesis cells centrifuge Chapter concentration containing cycle denaturation detection digestion DNA fragments DNA ligase DNA sequencing DNA templates dNTPs double-stranded DNA efficiency encoding ethanol exonuclease expression filters first-strand cDNA gel electrophoresis gene genomic DNA H₂O hybridization Incubate information panel Klenow fragment labeled length ligation mammalian method microfuge tube minutes molecules mRNA mutagenesis mutations Natl Nucleic Acids Nucleic Acids Res nucleotides oligonucleotide oligonucleotide primers PCR products plaques plasmid plasmid DNA plates polyacrylamide gel polymerase chain reaction preparation probes protein Protocol purified radioactivity radiolabeled reaction mixture reagents listed recombinant requires the reagents residues restriction enzyme reverse transcriptase RNase H room temperature screening sequencing reactions single-stranded DNA Step strand T4 DNA target DNA template DNA termini thermal cycler thermostable DNA polymerase tion transcription vector vitro µg/ml