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Molecular Cloning: Selected Applications in Medicine and Biology
Gregory G. Brown
Limited preview - 2011
activity addition agarose amount amplification Appendix appropriate bacteriophage bands base buffer carried cDNA libraries cells centrifuge Chapter chromatography cloning coli complete concentration constructed containing cycle denaturation described desired detection digestion Dilute DNA polymerase DNA sequencing dNTPs double-stranded efficiency electrophoresis enzyme et al ethanol expression filters four fragment gene genomic hybridization increase Incubate isolated labeled length ligation materials method minutes molecules mRNA mutagenesis mutations Nucleic Acids Res nucleotides oligonucleotide oligonucleotide primers panel plasmid plates position possible preparation presence primers probes problems protein Protocol purified radioactivity radiolabeled random reaction reagents recombinant region remove residues restriction enzyme reverse transcriptase samples screening single single-stranded solution specific Step stock solutions Store strain strand structure synthesis target DNA temperature template template DNA termini tion transcription transfer tube vector volume yield