Proteome Research: Two-Dimensional Gel Electrophoresis and Identification MethodsT. Rabilloud Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements. |
Contents
I | 1 |
II | 9 |
III | 10 |
IV | 14 |
V | 22 |
VII | 24 |
VIII | 27 |
IX | 31 |
XXXVIII | 144 |
XXXIX | 145 |
XL | 148 |
XLI | 149 |
XLII | 152 |
XLIII | 155 |
XLIV | 157 |
XLV | 159 |
X | 36 |
XI | 39 |
XII | 43 |
XIII | 47 |
XIV | 51 |
XV | 53 |
XVI | 57 |
XVII | 65 |
XVIII | 69 |
XIX | 80 |
XX | 82 |
XXI | 91 |
XXII | 93 |
XXIII | 99 |
XXIV | 107 |
XXV | 111 |
XXVI | 113 |
XXVII | 119 |
XXVIII | 123 |
XXIX | 124 |
XXX | 125 |
XXXI | 127 |
XXXII | 128 |
XXXIII | 132 |
XXXIV | 134 |
XXXV | 137 |
XXXVI | 139 |
XXXVII | 143 |
Other editions - View all
Proteome Research: Two-Dimensional Gel Electrophoresis and Identification ... T. Rabilloud No preview available - 1999 |
Proteome Research: Two-Dimensional Gel Electrophoresis and Identification ... T. Rabilloud No preview available - 2011 |
Common terms and phrases
2-D electrophoresis acrylamide amino acid amino acid analysis amino acid composition amino acid ratios amino acid sequence ammonium persulfate ampholytes Anal Biochem antibodies blotting buffer carrier ampholytes cassette cathodic cells Chem chemical cleavage concentration Coomassie database deionized water derivatization detection detergents digestion Edman Edman degradation electroblotted electrode Electrophoresis 18 equilibration ethanol extraction fluorescent Fmoc gene genome glass plates Görg hydrolysis hydrophobic IEF gel Immobiline in-gel IPG gel strips IPG strips isoelectric focusing labeling MALDI MALDI-MS mass spectrometry matrix method molecular weight molecules MS/MS N-terminal nitrocellulose nucleic acids peptide polyacrylamide gel polymerization polypeptides precipitation prepared procedure proteases protein identification proteome protocol PVDF Rabilloud reagent rehydration remove reswelling Rinse sample SDS gel SDS-PAGE second dimension sensitivity sequence tag silver staining slab gel sodium solubilization solution solvent spectra spots sulfate technique teins tion transfer tube two-dimensional electrophoresis two-dimensional gel electrophoresis urea voltage