Working with Animal ChromosomesA guide to handling giant polytene and lampbrush chromosomes, preparing chromatin, visualizing transcription, locating genes on chromosomes, identifing fine-scale polymorphisms on mitotic chromosomes, etc. Covers many specialized chromosome techniques; each method is tested and reproducible and comes with a model protocol. This 2nd Edition has several new sections on recently developed cytological techniques including spreading of synaptonemal complexes from cells in meiotic prophase, banding of mitotic chromosomes with Giemsa and fluorochromes, indirect immunofluorescence, lampbrush chromosomes from Xenopus laevis, and isolation of polytene chromosomes. Also covers new legislation affecting those who work with animal chromosomes. |
Other editions - View all
Common terms and phrases
acetic acid air dry amphibian animal antibodies autoradiography banding blood BrdU Callan cell suspension centrifuge centromere Chapter chromatin Chromosoma Berl chromosome preparations chromosome set clean colchicine concentration coverslip culture cytogenetics dispersal dissecting distilled water Drosophila melanogaster embryo cup emulsion ethanol Feulgen Figure filter paper fixation fixative fluorescence forceps G-banding gene Genet gently Giemsa grid human chromosomes hybridization hypotonic treatment incubation indirect immunofluorescence isolation karyotype labelled laboratory lampbrush chromosomes larvae lymphocytes Macgregor mammalian material medium meiosis meiotic metaphase metaphase chromosomes method microscope slide moist chamber newt nuclear nuclei nucleolus observation chamber oocyte orcein Pasteur pipette phase contrast phytohaemagglutinin Place the slides plethodontid polymerase polytene chromosomes probe procedure proteins protocol quinacrine remove resuspend ribosomal Rinse RNP fibrils room temperature salamanders salivary glands sequences species splash squash preparation staining subbed slide synaptonemal complexes technique testis tissue transcription units Triturus vial wash Xenopus µg/ml