The ABCs of Gene CloningClear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning. |
Contents
Structures of Nucleic Acids | 15 |
1 | 22 |
Organization of Genes | 39 |
6 | 55 |
Enzymes Used in Cloning | 68 |
8 | 77 |
9 | 92 |
Transformation | 125 |
Growth Enhancement in Transgenic Fish | 153 |
Finding DiseaseCausing Genes | 163 |
Human Gene Therapy | 171 |
Gene Targeting 179 | 178 |
DNA Typing | 187 |
Transpharmers Bioreactors for Pharmaceutical | 197 |
Animal Cloning | 201 |
References | 215 |
Isolating Genes for Cloning | 131 |
Transgenic Crops Engineered with Insecticidal Activity | 143 |
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Common terms and phrases
activity Agrobacterium alleles amino acid amino acid sequence animals bacteriophage baculovirus bands binds Biotechnol border sequence cDNA library CH₂ chain chromosome cloning vector coding codon coli construction contains DNA fragments DNA molecule DNA polymerase DNA sequence DNA strand encoding eukaryotic exon expression vectors foreign DNA Foreign gene functions gene cloning gene product gene sequence gene targeting genetic H₂N-CH-COOH homologous recombination host cell human hybridization infection inserted insulin introns isolated lac operon lactose lacZ membrane mRNA multiple cloning site mutation neo gene nuclear transfer nucleotide packaging pairs peptide PG gene phage plant cells plasmid vector polymorphic primer probe promoter protein provirus quence reaction reading frame recombinant DNA region repressor restriction enzymes retrovirus ribosome Section segment selectable marker ssDNA stem-loop structure substrate synthesis T-DNA Ti plasmid tion tissues tomato transcription transfection transformation transgenic translation viral DNA virus yeast