Parasite Antigens, Parasite Genes: A Laboratory Manual for Molecular Parasitology

Front Cover
Enthusiasts of immunological assays, protein chemistry and molecular cloning have a feast of guides and recipe books to follow. What the authors have achieved with this book has been to provide a more integrated reference work to reflect the arrival of molecular parasitology on the scientific scene, in which methods are presented concisely, together with essential explanations and background information. The aim has been not only to encourage the researcher with a defined plan in mind, but also to invite experienced workers to experiment in new areas and to overcome any difficulties new investigators may have in familiarising themselves with the field. Recent years have seen a dramatic expansion in the number of researchers into parasitic diseases, with an extraordinary diversity of scientific and medical backgrounds, and perhaps the broadest geographical distribution of any scientific field. The authors hope they will have provided to all members of this international research community a compact volume which will serve many purposes from initiation and execution of research projects, to learning and understanding more about these complex and fascinating biological organisms.
 

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Contents

Section I
1
Method
7
Carbohydrates
10
Antibodies
13
Method
19
Chemical Cleavage
87
19
90
Lectin Binding Assays
93
Hybridisation assays
141
In vitro Translation
149
Expression Libraries Method 41 cDNA Synthesis
151
59
159
Antibody Screening a gt11 Expression Library
165
Lysogeny and Fusion Protein Production from gt11
170
Subcloning into Plasmid Expression Vectors
172
Immunological Analysis with Recombinant Proteins
178

Gel Systems for Carbohydrate Analysis
96
Enzymes Method 30 Proteases and their Specificities
100
32
101
Protease Inhibitors
103
Detection of Proteases in Solution
105
Electrophoretic Separation of Proteolytic Activities
107
Enzyme Assays
109
44
113
Nucleic Acids Method 35 Isolation of Parasite RNA and DNA
114
Gel Electrophoresis and Restriction of DNA and RNA
121
48
123
Southern and Northern Blotting
131
Labelling DNA Probes
135
Section IO Recombinant DNA Method 47 Rapid Preparation of Recombinant Vector and Insert DNA
181
The Polymerase Chain Reaction PCR
184
DNA Sequencing
188
Construction and Screening of Genomic DNA Libraries
198
Appendices Appendix 1 Parasite Life Cycles and Culture
203
61
205
Buffers and Standard Solutions
206
Biochemical Data
210
64
216
Restriction Enzymes
217
79
223
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