Molecular Cloning: Selected Applications in Medicine and BiologyGregory G. Brown The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields. |
Contents
Principles | 21 |
Subcloning and Expression of Functional | 37 |
Molecular Cloning and Overexpression | 59 |
Effects of Two Novel Peptides | 73 |
Molecular Cloning of Immunoglobulin Heavy | 83 |
Identification of Molecules Involved in the Vulture | 91 |
Molecular Cloning Characterization | 109 |
Molecular Cloning Expression | 141 |
Molecular Cloning Expression Function | 197 |
Cloning the Ribokinase | 221 |
Genome Based Vaccines Against Parasites | 231 |
Phosphagen Kinase System of | 247 |
Molecular Cloning Expression Pattern | 267 |
Molecular Cloning and Characterization | 277 |
Cloning and Characterization of a Candidate Gene | 291 |
Strategies | 309 |
Other editions - View all
Molecular cloning: a laboratory manual, Volume 2 Joseph Sambrook,David W. Russell Limited preview - 2001 |
Common terms and phrases
a-WAP activity adelaida amino acid amino acid sequence amplified analysis Anosmin-1 antibodies antigens Arabidopsis arginine kinase assay B-cell bacterial binding Biochem Biol buffer cancer cathepsin Cathepsin K cDNA library cells characterization chromosome containing CroPrx3 CroPrx3-b dendrimers dendrosomes domain drug encoding enzyme epitopes expression vector Fe-SOD fluorescence fragments functional GenBank gene genetic genomic DNA human hybrid identified immune incubated induced inhibitors integrin interaction IPTG ISSN Journal ligands Loxosceles Loxosceles adelaida markers method Molecular Cloning molecules mRNA Mustafa Mycobacterium tuberculosis napus novel nucleotide palustrin-Ca peptide pernyi LysRS phage display phosphagen plants plasmid platelets polymerase polypeptide porcine CD41 predicted primers procathepsin proteomics protoplasts purified receptor recombinant proteins region residues ribokinase roseus RT-PCR screening SDS-PAGE selection SMase soluble specific Sphingomyelinase strategy structure studies substrate subunit Tambourgi target temporin-La tissues toxin transcripts transformation transmembrane tumor venom venom gland vulture TLR1 westermani