Introduction to FluorescenceThe phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understan |
Contents
1 Introduction | 1 |
2 Absorption of Light | 13 |
3 Instrumentation | 27 |
4 Emission and Excitation Spectra | 57 |
5 Polarization and Anisotropy | 75 |
6 TimeResolved Fluorescence | 101 |
7 Quantum Yields and Quenching | 131 |
8 Förster Resonance Energy Transfer | 145 |
9 Brief Overview of Fluorescence Microscopy | 163 |
10 Fluorophores | 191 |
11 Intrinsic Protein Fluorescence | 251 |
Rogues Gallery of Fluorescence Artifacts and Errors | 277 |
Back Cover | 286 |
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Common terms and phrases
absorbed absorption and emission absorption spectrum acid Additional Reading analogs anisotropy approach assays binding Biochem Biochemistry Biophys Chem components concentration confocal cuvette D.M. Jameson decay Depiction detector dimer dipole donor and acceptor dyes electronic emission spectra emitted energy transfer example excitation spectrum excitation wavelength exciting light extinction coefficient filter fluo fluorescein fluorescence lifetime fluorescence microscopy fluorescent proteins fluorophores FRET function Gregorio Weber hence illumination illustrated in Figure instrument intensity interactions intrinsic protein fluorescence labeled lamp laser ligand light source lipid living cells measurements membrane method modulation frequency molecular molecules monochromator monomer observed optical density orientation parameters phase and modulation phasor photobleaching photons polarization/anisotropy protein quantum yield quencher quenching Raman peak rhodamine rotational sample shown in Figure signal solution solvent spectrofluorimeter structure studies target tion tryptophan tryptophan residue typically tyrosine vitro wavelength Wavelength nm xenon arc lamp