Nonviral Vectors for Gene TherapyMien-Chie Hung, Leaf Huang, Ernst Wagner Gene transfer within humans has been an obstacle until about 10 years ago. At that time, it was found that viral vectors were effective carriers of "healthy genes" into patients' cells. The problem, however, was that viral vectors proved unnecessarily harmful to humans: subjects experienced inflamatory activity and negative immunological responses to the genes. Viral vectors were also unable to meet the needs of the pharmaceutical community: they were not reproducible in large-scale proportions in cost-effective ways. Thus, research was undertaken to find a safer way to transfer genes to patients without jeopardizing the safety of the patient. And so non-viral vectors were discovered. This volume presents the various non-viral vectors currently under development. Although not methodologically oriented, it will provide the necessary details behind the development of the vectors. This information will prove useful to both researchers and clinicians. Key Features * Presents state-of-the art developments of nonviral vectors as tools for modern molecular medicine * Covers all types of nonviral vectors, from molecular structure to therapeutic application Provides a comprehensive review of synthetic vectors * Includes contributions from major investigators and leading experts in the field |
Other editions - View all
Nonviral Vectors for Gene Therapy, Part 2 Leaf Huang,Mien-Chie Hung,Ernst Wagner Limited preview - 2005 |
Nonviral Vectors for Gene Therapy, Part 2 Leaf Huang,Mien-Chie Hung,Ernst Wagner No preview available - 2005 |
Common terms and phrases
Acad adenovirus administration airway animals anionic antibody antigen Behr binding Biochim Biol Biophys cationic lipid cationic liposomes cell lines cellular CFTR Chem chitosan CL/DNA complexes clinical cystic fibrosis cytokine cytoplasm DNA complexes DNA/liposome complexes dose DOTAP effect endocytosis endosomal endothelial cells endothelium enhance epithelium Felgner fluorescent gene delivery gene delivery systems gene expression gene gun gene therapy gene transfer genome Huang Human Gene Ther immune response increase induced injection interactions intravenous Lasic ligand lipoplex liposome complexes liposome/DNA complexes LPD-I luciferase lung mechanism mediated membrane mice molecular molecules mouse muscle naked DNA nanospheres Natl nuclear transport nucleic acid nucleus oncogene overexpression particles pDNA peptide plasmid DNA plexes polylysine potential Proc protein ratio receptor regulator release serum structure studies Szoka target gene tion tissue toxicity transfection transfection activity transfection efficiency transgene transgene expression vaccination viral virus vitro vivo gene Wang Zabner
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