Antibody Engineering Protocols

Front Cover
Sudhir Paul
Springer Science & Business Media, 1995 - Capillary electrophoresis - 450 pages
Capillary Electrophoresis Guidebook offers both newcomers and experienced research workers hands-on guidance to performing capillary electrophoresis. It provides sufficient practical advice to permit you to develop and optimize your own separations, along with extensive troubleshooting sections to overcome practical difficulties. The book contains operating instructions for standard commercially available instruments and includes guidelines for activities such as changing capillaries, method development, quantitative procedures, optimizing sensitivity, and the validation of methods. Review chapters written by leading experts discuss micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques, and electrochromatography. Important application areas such as the analysis of proteins, peptides, amino acids, pharmaceuticals, chiral compounds, and nucleic acids are also treated.
 

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Contents

SEQHUNT A Program to Screen Aligned Nucleotide and Amino Acid Sequences
2
Molecular Modeling of AntibodyCombining Sites
17
Structure and Properties of Human Immunoglobulin LightChain Dimers
51
Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins
83
Detection of Human Variable Gene Family Expression at the SingleCell Level
99
Purification of Reduced and Alkylated Antibody Subunits
111
Murine Monoclonal Antibody Development
123
Comparative Properties of Polyclonal and Monoclonal Antibodies
139
Expression of Chimeric Immunoglobulin Genes in Mammalian Cells
251
SingleChain AntiDNA Fv
265
Molecular Cloning of AntigroundState Proteolytic Antibody Fragments
281
Cloning and Bacterial Expression of an Esterolytic sFv
297
SiteDirected Mutagenesis of AntibodyVariable Regions
319
Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation
329
Chaperonins in Phage Display of Antibody Fragments
343
PhageDisplay Libraries of Murine and Human Antibody Fab Fragments
355

Evaluation of Antibody Clonality
151
Affinity Immunoblotting
165
Epitope and Idiotope Mapping Using Monoclonal Antibodies
171
AntiIdiotypic Antibodies That Mimic Opioids
183
Catalytic Antibodies Structure and Possible Applications
203
Preparation and Assay of Acetylcholinesterase Antibody
211
DNA Hydrolysis by Antibodies
223
Screening Strategies for Catalytic AntitransitionState Analog Antibodies
237
Selection of Human Immunoglobulin Light Chains from a PhageDisplay Library
377
Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
395
Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening
403
Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies
409
Methods of Measuring Thyroglobulin and PeptideMethylcoumarinamide Hydrolysis by Autoantibodies
417
Radiolabeling of Antibodies for Therapy and Diagnosis
423
Index
441
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