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can be used to examine
used for detection
II and Appendix III
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acid activity added addition affinity allow amino acids amount anti antigen Appendix approach appropriate background bands beads binding blocking blot bodies bound buffer Caution cells changes chemical choice collected column common commonly compared complex concentration contain coupling cross-reactions detection determine dilutions elution enzyme epitopes example expression final fixed gene give glasses immunoblotting immunoglobulin immunoprecipitation immunostaining Incubate interactions labeled levels light lower membrane method minutes molecular molecules monoclonal antibodies multiple needed normally peptide Place plates possible preparation presence primary antibody problems procedure protein purification reaction recognize recommend regions relative remove room temperature samples secondary reagents sensitivity sequence serum signal slides sodium solution specific specimen staining step stored structure supernatant Table techniques tion tissue transfer Tris tube volume Wash
Page 31 - Benjamin DC, Berzofsky JA, East IJ, Gurd, FRN, Hannum C., Leach SJ, Margoliash E., Michael JG, Miller A., Prager EM, Reichlin M., Sercarz EE, Smith-Gill SJ, Todd PE, and Wilson AC 1984. The antigenic structure of proteins: A reappraisal. Annu. Rev. Immunol. 2: 67-101.
Page 424 - Davis, BJ (1964) Disc electrophoresis — II. Method and application to human serum proteins.
Page 335 - K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gin; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; Y, Тут.
Page 10 - Segal, DM, Padlan, EA, Cohen, GH, Rudikoff, S., Potter, M., and Davies, D. R. (1974) The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site. Proc. Natl. Acad. Sci. USA 71, 4298^302.
Page 424 - Laskey, RA and Mills, AD (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
Page 424 - Bonner, WM, and Laskey, RA (1974) A film detection method for tritiumlabelled proteins and nucleic acids in polyacrylamide gels.
Page 32 - Amit AG, Mariuzza RA, Phillips SEV, and Poljak RJ 1986. Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution.
Page 15 - Alt, FW/ Yancopoulos, GD/ Blackwell/ TK, Wood/ C./ Thomas/ E., Boss/ M./ Coffman/. R./ Rosenberg/ N., Tonegawa/ S. and Baltimore/ D.